Roy Zito is the thirty-five year old underboss of the Gambetti Family, a role he took over when Sammy Bottino went into hiding. Zito runs a number of Gambetti owned dry cleaners throughout Liberty City that specialize in removing forensic evidence. Fiercely loyal to his Don; rumor has it that when Jon Gravelli was facing kidney failure Zito offered him his kidney. Gravelli refused the offer but Zito countered by removing his own kidney with a knife and delivering it to Gravelli in a bucket of ice. He is most likely to be the successor to Gravelli. Tony Prince mentions that he knows from "personal experience" that Roy Zito is a closeted homosexual, although if asked he would say Tony spiked his drink.
Controls several legitimate business including a chain of dry cleaners that specialize in removing forensic evidence from clothing.
Believed to have offered one of his kidneys to the ailing Jon Gravelli in tribute. When Gravelli refused the offer, it is reported that Zito cut the kidney out himself and gave it to the boss in a bucket of ice.