The Vice City Mambas are Vice City's football team in the GTA III Era. BJ Smith and Jose Pimsoll used to play with the Mambas. In the mission Two-Hand Toss in Grand Theft Auto Advance, protagonist Mike has to injure a Mambas quarterback to improve the chances of the Liberty City Cocks to win, on orders from Asuka Kasen.

In Grand Theft Auto: San Andreas, Vice City Mambas posters can be seen inside the Welcome Pump in Dillimore.