Lone Wolf Biker is a multiplayer mode in The Lost and Damned.
In this mode, the player starts as "The Lone Wolf", who is on the run while everyone else is out to get them. The Lone Wolf must go through a series of checkpoints, while the rest of the participants attempt to kill them and take over the role of Lone Wolf. If the Lone Wolf is killed, the person that kills them takes over. Passing through checkpoints gives the Lone Wolf pipe bombs and keeps the clock ticking. Avoiding or failing to reach checkpoints within a certain amount of time prevents the Lone Wolf from accumulating time until a checkpoint is reached.
The player limit is from 2 to 16 members.